Biological Information

Family:

Epigenetic

Sub Family:

Histone Methyltransferase (HMT)

Class:

Writer

Protein Name:

ASH1L

Uniprot Number:

Q9NR48

Gene Name:

ASH1L

Gene ID:

55870

Gene Aliases:

huASH1|ASH1|ASH1L1|KMT2H

Accession Number:

NM_018489

Organism:

Human

Construct Details:

Recombinant

Assay Information

Assay Type:

Biochemical

Assay Sub Type:

Enzymatic

Functional Mode:

Antagonist

Detection Method:

Radiometric

Measured Response:

Scintillation

Testing Information

Substrate:

Polynucléosome (1.5 µg/ml)

Tracer:

[3H] SAM (150nM)

Incubation:

15 min at RT

Control Inhibitor:

SAH

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.

Test Sample Requirements:

Minimum for 1) Screen: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 μl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.

Minimum Order Quantity:

10

Turnaround Time

Standard:

15 Business Days

Reference Compound Data

PubChem ID

65482

Name

Sinefungin

Measurement

6.7µM - IC50

PubChem ID

11657687

Name

Chaetocin

Measurement

5.3µM - IC50

PubChem ID

25246222

Name

SAH

Measurement

7.7µM - IC50

PubChem ID

73357457

Name

935693-62-2

Measurement

97µM - IC50

Additional Information

Brand:

LeadHunter

Testing Location:

France - Celle L'Evescault