Biological Information

Background Information:

Cathepsin C or CTSC, also known as dipeptidyl aminopeptidase I (DPPI) or dipeptidyl transferase, is a lysosomal cysteine peptidase belonging to the papain family. Human tissues show that spleen and kidney are the richest sources of cathepsin C. In addition, serum levels are highest in hepatic diseases, followed by peripheral arterial disease, thromboembolism, myocardial infarction, diabetes mellitus, and prostatic hypertrophy. Also, cathepsin C activity is present at higher levels in cytotoxic lymphocytes and myeloid cells.

Family:

Protease

Sub Family:

Papain

Class:

Cysteine Peptidase

Protein Name:

Cathepsin C

Uniprot Number:

P53634

Protein Aliases:

Cathepsin C, Cathepsin J, Dipeptidyl peptidase I?(DPP-I;?DPPI), Dipeptidyl transferase

Gene Name:

CTSC

Gene ID:

1075

Gene Aliases:

DPP1

Accession Number:

NM_001814.6

Organism:

Human

Construct Details:

Fragment, recombinant

Assay Information

Assay Type:

Biochemical

Assay Sub Type:

Enzymatic

Functional Mode:

Antagonist

Detection Method:

Spectrofluorimetry

Measured Response:

Fluorescence

Testing Information

Procedure Summary:

Human cathepsin C expressed in a mouse myeloma cell line is used. Test compound and/or vehicle is preincubated with 2 μg/ml of active enzyme in modified MES buffer pH 6.0 for 15 minutes at 25°C. The reaction is initiated by addition of 20 μM Gly-Arg-AMC for another 30-minute incubation period. Determination of the amount of AMC formed is read spectrofluorimetrically at 360 nm/465 nm. Compounds are screened at 10 μM.

Substrate:

20 µM Gly-Arg-AMC

Incubation:

30 min at 25°C

Control Inhibitor:

E-64

Criteria For Significance:

≥ 50% of max stimulation or inhibition

Test Concentration / Dose:

Suggested concentration for initial testing is 10 uM

Test Sample Requirements:

Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).

Minimum Order Quantity:

2

Turnaround Time

Standard:

15 Business Days

Reference Compound Data

PubChem ID

11957542

Name

E-64

Measurement

0.36μM - IC50

Clinical Relevance

Therapeutic Area:

Cardiovascular

Additional Information

Brand:

LeadHunter

Testing Location:

Taiwan - Taipei

RELATED SOLUTIONS

Cathepsin LeadHunter Panel - TW
Item: PP326
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