Cathepsin Z Human Papain Cysteine Peptidase Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
CTSZ (Cathepsin Z, also known as cathepsin P and X or CTSX) is a cysteine protease in the papain family. It is ubiquitously expressed in human tissues and conserved in many species. Acting as a carboxypeptidase, cathepsin Z displays a unique specificity. It is ubiquitously expressed in human tissues and also distributed in cancer cells and primary tumors from different sources. Inhibition of Cathepsin Z may inhibit tumorigenesis.
Family:
Protease
Sub Family:
Papain
Class:
Cysteine Peptidase
Protein Name:
Cathepsin Z
Uniprot Number:
Q9UBR2
Protein Aliases:
cathepsin X|carboxypeptidase LB|cathepsin IV|cathepsin B2|cathepsin Y|cathepsin Z1|cysteine-type carboxypeptidase|lysosomal carboxypeptidase B
Gene Name:
CTSZ
Gene ID:
1522
Gene Aliases:
CTSX
Accession Number:
NM_001336
Organism:
Human
Construct Details:
Full Length, Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human recombinant cathepsin Z expressed in murine myeloma NS0 cells is used. Test compound and/or vehicle is preincubated with 0.08 μg/ml enzyme‡ in modified acetate buffer pH 3.5 for 15 minutes at 25⁰C. The reaction is initiated by addition of 10 μM Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH for another 60 minute incubation period. Determination of the amount of Mca-Arg formed is read spectrofluorimetrically at 320 nm/405 nm. Compounds are screened at 10 μM.
Substrate:
10 µM Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH
Incubation:
60 min at 25°C
Control Inhibitor:
Leupeptin
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
RELATED SOLUTIONS
Cathepsin LeadHunter Panel - TW
Item: PP326
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