Biological Information
Background Information:
Cannabinoid receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Cannabinoid CB2 receptors are found primarily in immune tissues.
Family:
GPCR
Sub Family:
Cannabinoid
Class:
Class A
Protein Name:
CB2
Uniprot Number:
P34972
Protein Aliases:
CB2|hCB2|CX5
Gene Name:
CNR2
Gene ID:
1269
Gene Aliases:
CB2
Accession Number:
NM_001841
Organism:
Human
Construct Details:
Recombinant
Cell Type:
CHO-K1
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Antagonist
Detection Method:
HTRF
Testing Information
Incubation:
10 min at 37°C
Control Inhibitor:
AM 630
Control Activator:
WIN 55,212-2 mesylate (3 nM)
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µL of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM finµL testing. 2) Dose Response: 90 µL of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
16
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Adverse / Beneficial Action:
Cannabinoid CB2 receptor agonism may limit angiogenesis as well as induce apoptosis in some types of cancer, inhibit inflammatory pain and the immune system. CB2 receptor antagonism may facilitate mast cell-mediated allergic and inflammatory responses.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
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