Time-dependent CYP1A Inhibition (HLM; phenacetin substrate) - US
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Cytochrome P450 (CYP) inhibition is one of the most common types of drug-drug interactions (DDI) and can be exhibited in either a reversible or time-dependent manner. This assay is designed to determine if a test compound is an irreversible, time-dependent inhibitor of CYP1A in human liver microsomes
No. of Assays on Panel:
2
Assays on Panel:
Family
CYP450
Name
Time-dependent CYP1A inhibition (HLM, phenacetin substrate + NADPH) - US
Item
2634
Protein
CYP1A2
Organism
Human
Assay Sub Type
Enzymatic
Family
CYP450
Name
Time-dependent CYP1A inhibition (HLM, phenacetin substrate - NADPH) - US
Item
2635
Protein
CYP1A2
Organism
Human
Assay Sub Type
Enzymatic
Family:
CYP450
Sub Family:
CYP1
Protein Name:
CYP1A1
Uniprot Number:
P04798
Gene Name:
CYP1A1
Gene ID:
1543
Gene Aliases:
P450DX|P1-450|P450-C|CP11
Organism:
Human
Construct Details:
Microsomes
Tissue:
Liver
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
HPLC-MS/MS
Measured Response:
Peak Area Response
Testing Information
Substrate:
phenacetin
Test Concentration / Dose:
Assay is performed with each concentration tested using 2 replicate(s)
Test Sample Requirements:
Minimum for 1) Screen: 100 μl of 10 mM stock -OR- 1 to 2 mg (pre-weighed). 2) Dose Response: 250 μl of 10 mM stock -OR- 1.5 to 2 mg (pre-weighed).
Turnaround Time
Standard:
10 Business Days
Additional Information
Brand:
Cerep
Testing Location:
USA - St Charles, MO
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