In Vitro and In Vivo Discovery Platforms
Antibody discovery uses in vitro library screening and in vivo immunization strategies to generate and isolate antibodies that can modulate the activity of a specific target involved in the pathophysiology of a disease. One or a combination of these techniques enables the discovery of unique therapeutic antibody candidates, which can then be further/fully characterized for desirable affinity, specificity, function, and developability characteristics.
Many Options for Antibody Discovery
At Eurofins Discovery, we rely on multiple discovery platforms, including immunization of different species or multiple display technologies, to provide you with flexible options to generate the most promising antibody candidates.
In Vivo Immunization
In vivo immunization involves the introduction of a target antigen into an animal, such as a mouse or rabbit, to stimulate a natural immune response, producing antibodies. Various transgenic animal models have been developed to generate humanized, single-chain, or multi-specific antibodies. Eurofins Discovery can use your license for a humanized animal campaign. The multitudes of antibodies from these immunized animal platforms can be recovered and analyzed to identify antigen-specific antibodies. Eurofins Discovery leverages a broad portfolio of animal models to generate unique, high-quality antibody candidates.
- Native animal platforms
- Humanized animal platforms
Hybridoma and Single B-Cell Recovery
Following in vivo immunization, isolating antibody-producing cells is accomplished using hybridoma techniques and single B-cell recovery. Hybridomas are generated by immortalizing antibody-producing spleen or lymph node cells, while antigen-specific single B-cells can be isolated and sequenced. Hybridomas and single B-cells can be used as a reliable source of antigen-specific antibodies for further characterization. Our team uses state-of-the-art instrumentation and decades of experience to deliver the most promising antibody candidates.
In Vitro Display
Phage, yeast, and mammalian display techniques use synthetic or naturally-derived sequences expressed in Fab or scFv formats to enrich for antigen-specific candidates. Through multiple rounds of panning, display techniques can generate specific, ideal candidates. Eurofins Discovery can provide off-the-shelf or custom display libraries in various formats to ensure the successful selection of highly specific candidates.
- Naive libraries
- Immune libraries
