Target Validation
Target validation is a key first step in biotherapeutics discovery. Using functional in vitro, in vivo, and/or biochemical assays confirms that pharmacological perturbation of a molecular target leads to disease efficacy, building a case for additional preclinical studies and a stronger probability of success in downstream clinical testing.
Build Confidence in the Right Target with the Right Team
At Eurofins Discovery, we develop and validate cell-based assays so you can validate your molecular target and offer a first glimpse at efficacy, safety, toxicity, pharmacokinetics (PK), and metabolism.
Assay Development
Reagent development, Assay design, and evaluation of a tool molecule, if available, are essential for proving the validity of a drug concept.
Antigen Generation
Antigens are essential reagents for any antibody discovery program, whether using an in vitro or in vivo discovery platform. Recombinant antigens are expressed transiently or stably in cell lines, purified, characterized and used for animal immunization or in vitro display technologies. Maintaining the quality of these critical reagents and the cells that produce them is vital for a successful antibody discovery campaign.
Critical Reagent Generation from Biologics Experts
Eurofins Discovery ensures high-quality protein, cell line, DNA, and other reagents are maintained over the lifespan of your discovery program.
Transient and Stable Expression Systems
There are two methods for producing antigens or other recombinant proteins for an antibody or other biotherapeutic discovery program: transient expression, which involves rapid, repeatable small-scale production, or stable expression, which involves long-term, steady-state or large-scale production. Eurofins Discovery assists you in choosing the best bacterial, insect, or mammalian expression system for your target protein quantity and quality.
Protein Production
Protein expression, purification, and characterization are necessary for immunization, in vitro selection, or biochemical assays. Each protein can have a complex purification process or nuanced storage conditions that must be determined for a reliable discovery program. Our team offers comprehensive recombinant protein engineering, expression, and purification expertise for both routine and challenging protein targets.
Reagent Qualification and Banking
Reagent qualification and banking is the generation, characterization and maintenance of high-quality reagents such as proteins, cell lines, and DNA – to ensure they remain well-defined throughout preclinical development. Reliable reagents are essential for successful immunization, screening, functional characterization, and optimization, leading to potent, safe, and selective biologics. Eurofins Discovery offers high-quality protein production, and cell line generation services, ensuring reagent quality through regular testing to support your entire biotherapeutics discovery effort.
