H3 Human Histamine GPCR Binding Agonist Radioligand LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Histamine receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Histamine receptors are found in most peripheral tissue and within the central nervous system. Histamine H3 receptors on nerve terminals function as feedback inhibitors in a wide variety of organ systems.
Family:
GPCR
Sub Family:
Histamine
Class:
Class A
Protein Name:
H3
Uniprot Number:
Q9Y5N1
Protein Aliases:
HH3R|G-protein coupled receptor 97
Gene Name:
HRH3
Gene ID:
11255
Gene Aliases:
GPCR97
Accession Number:
NM_007232
Organism:
Human
Construct Details:
Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Binding
Detection Method:
Radiometric
Measured Response:
Scintillation
Testing Information
Ligand:
[3H]Nalpha -Me-histamine
Ligand Kd (nM):
0.32
Ligand Concentration:
1 nM
Non Specific:
1 µM (R)alpha -Me-histamine
Incubation:
60 min at RT
Control Inhibitor:
(R)-alpha-Methylhistamine
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 μl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
2
Turnaround Time
Standard:
10 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Adverse / Beneficial Action:
Histamine H3 receptors are presynaptic receptors present on histaminergic nerve terminals in the CNS that exert feedback regulation of histamine synthesis and release.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
H3 Human Histamine GPCR Cell Based Agonist cAMP LeadHunter Assay - FR
Item: 1800
H3 Human Histamine GPCR Cell Based Antagonist cAMP LeadHunter Assay - FR
Item: 1801
H3 Human Histamine GPCR Cell Based Agonist & Antagonist cAMP LeadHunter Assay - FR
Item: G021
BioPrint SafetyScreen Panel - FR
Item: P22-p
Diversity LeadHunter Panel - FR
Item: P9
CNS SafetyScreen panel - FR
Item: P411
Anti-Epileptic Discovery LeadHunter Panel - FR
Item: P427
Questions? Contact Us
You can easily get in touch with a Eurofins Discovery Expert or get help from our Customer Service team. Reach out with questions, or to discuss your specific discovery challenges.
