OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Evaluation of two compounds in any BioMAP system, individually and in combination. The StroHT29 system is comprised of dermal fibroblasts, the HT-29 colorectal adenocarcinoma cell line, and peripheral blood mononuclear cells. This stromal tumor microenvironment (TME) model is stimulated via the T cell receptor for 48-hrs to recapitulate the activation of tumor infiltrating immune cells in the context of an immunosuppressive cancer cell line and normal host stromal tissue. These conditions model the host stromal-immune-tumor microenvironment by capturing the complex interactions between tumor cells, the host stromal network, and infiltrating immune cells recruited into the tumor mass. Biomarker readouts capture impacts on translationally relevant immune, matrix remodeling, and inflammation-related intratumoral responses.
Organism:
Human
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Testing Information
Test Concentration / Dose:
Two test agents profiled alone at four concentrations, and in combination in a four-by-four matrix, in triplicate.
Test Sample Requirements:
For Small Molecules: 350uL of a 1000X stock based on the top concentration being tested & standard 3-fold dilutions per system and per Combo. For Biologics: 3.5mL of a 100X stock in PBS or water, based on the top concentration being tested & standard 3-fold dilutions per system and per Combo
Turnaround Time
Standard:
40 Business Days
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
BioMAP
Testing Location:
USA - St Charles, MO
RELATED SOLUTIONS
BioMAP Systems ELECT Menu 3 (StroHT29)
Item: 89-0003-0045
BioMAP Combo ELECT Menu 3 (StroC)
Item: 89-0015-0047
BioMAP RNA ELECT (StroHT29)
Item: 89-0035-0045
BioMAP Oncology Panel (CRC)
Item: 89-0044-0901
OncoPanel® Proliferation Assay in HT-29 Cells, 3-day Multi-Parameter High Content Imaging
Item: HT2MP03
OncoPanel® Proliferation Assay in HT-29 Cells, 5-day Multi-Parameter High Content Imaging
Item: HT2MP05
OncoPanel® Proliferation Assay in HT-29 Cells, 10-day Multi-Parameter High Content Imaging
Item: HT2MP10
OncoPanel® Proliferation Assay in HT-29 Cells, 3-day Singleplex High Content Imaging
Item: HT2SP03
OncoPanel® Proliferation Assay in HT-29 Cells, 5-day Singleplex High Content Imaging
Item: HT2SP05
OncoPanel® Proliferation Assay in HT-29 Cells, 10-day Singleplex High Content Imaging
Item: HT2SP10
OncoPanel® 3D Spheroid Viability Assay in HT-29 Cells
Item: HT2SP3D
OncoPanel®, Full 3D Spheroid Panel, with 2D Control, Cell Viability
Item: PSOP0001
OncoPanel®, Full 3D Spheroid Panel, without 2D Control, Cell Viability
Item: PSOP0002
OncoPanel®, Full Panel: Custom Assay
Item: PSOP0003
OncoPanel®, Full Panel: High Content Imaging Cell Proliferation Assay, 10-day long-term
Item: PSOP0010
OncoPanel®, Full Panel: High Content Imaging Cell Proliferation Assay, 3-day
Item: PSOP0011
OncoPanel®, Full Panel: High Content Imaging Cell Proliferation Assay, 5-day
Item: PSOP0012
OncoPanel®, Full Panel: Multi-Parameter High Content Profiling Assay, 10-day long-term
Item: PSOP0013
OncoPanel®, Full Panel: Multi-Parameter High Content Profiling Assay, 3-day
Item: PSOP0014
OncoPanel®, Full Panel: Multi-Parameter High Content Profiling Assay, 5-day
Item: PSOP0015
Questions? Contact Us
You can easily get in touch with a Eurofins Discovery Expert or get help from our Customer Service team. Reach out with questions, or to discuss your specific discovery challenges.
