OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Evaluation of two compounds in any BioMAP system, individually and in combination. The VascHT29 system is comprised of venular endothelial cells, the HT-29 colorectal adenocarcinoma cell line, and peripheral blood mononuclear cells. This vascular tumor microenvironment (TME) model is stimulated via the T cell receptor for 48 hrs to recapitulate the activation of tumor infiltrating immune cells in the context of an immunosuppressive cancer cell line and normal host vascular tissue. These conditions model the host vascular-immune-tumor microenvironment by capturing the complex interactions between tumor cells, the host vascular network, and infiltrating immune cells associated with angiogenesis. Biomarker readouts capture impacts on translationally relevant immune, angiogenesis, and inflammation-related intertumoral responses.
Organism:
Human
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Testing Information
Test Concentration / Dose:
Two test agents profiled alone at four concentrations, and in combination in a four-by-four matrix, in triplicate.
Test Sample Requirements:
For Small Molecules: 350uL of a 1000X stock based on the top concentration being tested & standard 3-fold dilutions per system and per Combo. For Biologics: 3.5mL of a 100X stock in PBS or water, based on the top concentration being tested & standard 3-fold dilutions per system and per Combo
Turnaround Time
Standard:
40 Business Days
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
BioMAP
Testing Location:
USA - St Charles, MO
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