Biological Information

Background Information:

Neuropeptide Y receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Neuropeptide Y2 receptors are found primarily, but not entirely, in the central nervous system.

Family:

GPCR

Sub Family:

Neuropeptide Y

Class:

Class A

Protein Name:

Y2

Uniprot Number:

P49146

Protein Aliases:

NPY-Y2 receptor|Y2 receptor

Gene Name:

NPY2R

Gene ID:

4887

Accession Number:

NM_000910

Organism:

Human

Assay Information

Assay Type:

Biochemical

Assay Sub Type:

Binding

Detection Method:

Radiometric

Measured Response:

Scintillation

Testing Information

Ligand:

[125I]peptide YY

Ligand Kd (nM):

0.01

Ligand Concentration:

0.015 nM

Non Specific:

1 µM NPY

Incubation:

60 min at 37°C

Control Inhibitor:

NPY

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.

Test Sample Requirements:

Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.

Minimum Order Quantity:

2

Turnaround Time

Standard:

15 Business Days

Reference Compound Data

PubChem ID

Name

NPY

Measurement

0.099nM - IC50

PubChem ID

Name

NPY-(13-36)

Measurement

0.92nM - IC50

PubChem ID

Name

[Leu31,Pro34]NPY

Measurement

397nM - IC50

Clinical Relevance

Therapeutic Area:

CNS/Addiction/Pain

Adverse / Beneficial Action:

Neuropeptide Y2 receptor agonism may reduce sympathetic nerve output and cause colonic spasm. Neuropeptide Y2 receptor antagonism may be useful in the treatment of alcoholism.

Additional Information

Brand:

LeadHunter

Testing Location:

France - Celle L'Evescault

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