V1B Human Vasopressin / Oxytocin GPCR Binding Agonist Radioligand LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Vasopressin receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Vasopressin receptors are currently classified into V1A, V1B (V3) and V2 subtypes. The V1B receptor is known to be present only in the adenohypophysis.
Family:
GPCR
Sub Family:
Vasopressin and Oxytocin
Class:
Class A
Protein Name:
V1B
Uniprot Number:
P47901
Protein Aliases:
AVPR V1b|AVPR V3|Antidiuretic hormone receptor 1b|Vasopressin V3 receptor
Gene Name:
AVPR1B
Gene ID:
553
Gene Aliases:
AVPR3|VPR3
Accession Number:
NM_000707
Organism:
Human
Construct Details:
Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Binding
Detection Method:
Radiometric
Measured Response:
Scintillation
Testing Information
Ligand:
[3H]AVP
Ligand Kd (nM):
0.2
Ligand Concentration:
0.2 nM
Non Specific:
0.1 µM AVP
Incubation:
120 min at RT
Control Inhibitor:
Vasopressin
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
CNS/Addiction/Pain
Adverse / Beneficial Action:
Vasopressin V1B receptor antagonism may inhibit ACTH release and be useful in the treatment of anxiety and depression.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
V1B Human Vasopressin / Oxytocin GPCR Cell Based Agonist Calcium Flux LeadHunter Assay - FR
Item: 4312
V1B Human Vasopressin / Oxytocin GPCR Cell Based Antagonist Calcium Flux LeadHunter Assay - FR
Item: 4313
V1B Human Vasopressin / Oxytocin GPCR Cell Based Agonist & Antagonist Calcium Flux LeadHunter Assay - FR
Item: G296
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