Biological Information

Background Information:

Androgen receptors are members of a superfamily of nuclear receptors that transduce the effects of a diverse array of small, hydrophobic ligands, including the steroid hormones, thyroid hormone, vitamin D and retinoids.

Family:

NHR

Sub Family:

3-Ketosteroid

Class:

Steroid

Protein Name:

AR

Uniprot Number:

P10275

Protein Aliases:

testicular feminization|Kennedy disease

Gene Name:

AR

Gene ID:

367

Gene Aliases:

AIS|NR3C4|SMAX1|HUMARA

Organism:

Human

Assay Information

Assay Type:

Biochemical

Assay Sub Type:

Binding

Detection Method:

Radiometric

Measured Response:

Scintillation

Testing Information

Ligand:

[3H]methyltrienolone

Ligand Kd (nM):

0.6

Ligand Concentration:

1 nM

Non Specific:

1 µM Testostérone

Incubation:

240 min at 22°C

Control Inhibitor:

Testosterone

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.

Test Sample Requirements:

Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.

Minimum Order Quantity:

2

Turnaround Time

Standard:

15 Business Days

Reference Compound Data

PubChem ID

5994

Name

Progesterone

Measurement

5.8nM - IC50

PubChem ID

24278426

Name

17-beta -estradiol

Measurement

21nM - IC50

PubChem ID

329769469

Name

Testosterone

Measurement

5.4nM - IC50

PubChem ID

24894947

Name

Fluoxymesterone

Measurement

55nM - IC50

Clinical Relevance

Therapeutic Area:

Metabolic Diseases

Adverse / Beneficial Action:

Testosterone receptor agonists may promote prostate cancer and cause edema while antagonists may inhibit spermatogenesis and be useful in the treatment of prostate cancer.

Additional Information

Brand:

LeadHunter

Testing Location:

France - Celle L'Evescault

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