Biological Information
Background Information:
Androgen receptors are members of a superfamily of nuclear receptors that transduce the effects of a diverse array of small, hydrophobic ligands, including the steroid hormones, thyroid hormone, vitamin D and retinoids.
Family:
NHR
Sub Family:
3-Ketosteroid
Class:
Steroid
Protein Name:
AR
Uniprot Number:
P10275
Protein Aliases:
testicular feminization|Kennedy disease
Gene Name:
AR
Gene ID:
367
Gene Aliases:
AIS|NR3C4|SMAX1|HUMARA
Organism:
Human
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Binding
Detection Method:
Radiometric
Measured Response:
Scintillation
Testing Information
Ligand:
[3H]methyltrienolone
Ligand Kd (nM):
0.6
Ligand Concentration:
1 nM
Non Specific:
1 µM Testostérone
Incubation:
240 min at 22°C
Control Inhibitor:
Testosterone
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Adverse / Beneficial Action:
Testosterone receptor agonists may promote prostate cancer and cause edema while antagonists may inhibit spermatogenesis and be useful in the treatment of prostate cancer.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
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