Leverage high-performance kinase activity screening with Eurofins Discovery’s KinaseProfiler™, the gold-standard platform trusted by leading pharmaceutical and biotechnology companies worldwide.
We assess kinase activity, selectivity, and support early derisking, from individual kinase assays to large-scale panels. We deliver high-quality controlled assays with the data accuracy, rapidity, and scientific rigor you need to accelerate your drug discovery program.
KinaseProfiler Key Features
- Kinase coverage: 450+ biologically relevant kinases, including lipid kinases
- Assay technologies: Radiometric, luminescence, TR-FRET
- Flexible formats: Single-point, multi-point, 9-point IC50
- ATP concentration: 10 µM or Km
- Turnaround time: 6 business days (guaranteed)
- Reliability: Batch-tested proteins with IC50 validation, <1% error rate
- Adaptability: customized subscription agreement in sub-panel, volume and time
- Data visualization: Kinome activity mapping available via TREEspot™
TREEspot enables rapid selectivity assessment, compound triaging, and off-target risk evaluation in a single kinome-wide view.
(NEW!) Our KinaseProfiler offering is continuously expanding to ensure optimal coverage of emerging and high-interest targets as well as support evolving discovery programs. This includes:
- TNK1 and MAST4, with emerging genetic links to cognitive phenotypes.
- EPHA6, involved in neuronal and synaptic signaling.
- RIPK5, connected to inflammatory and cell-death–related signaling pathways.
- PDHK1, linking kinase activity to mitochondrial metabolism.
KinaseProfiler Detection Technologies
Eurofins Discovery offers three complementary assay formats – radiometric (filter binding), luminescence (ADP detection), and TR-FRET (HTRF®) – to ensure optimal coverage across diverse kinase families.
| Predefined Panel | Number of Kinases | ATP Options | Testing Conditions | TAT |
|---|---|---|---|---|
| Full Kinase Panels | 452 | 10µM*, Km** | 1 to 9 concentrations | 6 Business Days |
| Full Human Kinase Panels | 435 | 10µM*, Km** | 1 to 9 concentrations | |
| Full Human WT Kinase Panels | 395 | 10µM*, Km** | 1 to 9 concentrations | |
| Diversity Kinase Panels | 58 | 10µM*, Km** | 1 to 9 concentrations |
* Panels tested at 10 µM ATP are run on a scheduled basis. Run dates are available in the Documents/References tab of each 10µM panel page.
** For Km-based assays, ATP concentration is set around the kinase Km ±15 µM, with a minimum of 10 µM.
FAQ about KinaseProfiler:
- Why should I run a functional kinase panel?
- To measure how your compound inhibits kinase activity under relevant ATP conditions and generate reliable IC50 values.
- This is essential for:
- Ranking compounds and driving SAR
- Understanding mechanism of action
- Generating robust, decision-ready data at scale
- Which ATP condition should I choose (Km or 10 µM)?
- It depends on your objective:
- Km → best for comparing compounds and detecting ATP-competitive inhibitors
- 10 µM → high sensitivity for early hit detection
- When should I use a broad panel versus a focused panel?
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- Broad panels are essential when assessing selectivity, especially before candidate selection.
- Focused panels are more appropriate during lead optimization, offering a more cost-efficient approach for SAR on known targets or defined kinase families.
- When is single-point profiling sufficient vs IC50 curves?
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- Single-point (1cc) → best for fast triaging and compound prioritization
- IC50 curves → required for accurate potency ranking and SAR decisions
IC50 becomes critical as soon as you compare compounds across programs or campaigns.
Complementary Solutions for Kinase Profiling
Boost Your Lead Compound with Kinase BRET Cellular Profiling
Advance your profiling in cellular environment with our BRET Kinase Target Engagement assays, available across a portfolio of 350+ full-length kinases, including mutants This technology helps improve lead compound success by measuring affinity for the intended kinase target in a cellular environment, supporting confident progression to the next stage of drug discovery.
- ✓ Real-time affinity assessment in intact cells
- ✓ Robust results with an average of Z’ = 0.8
- ✓ Competitive pricing & 15-business-day TAT
- ✓ Standardized or customizable assays
Deepen Your Understanding with Customizable Solutions
Whether you’re working on novel kinases, rare mutations, or unique assay conditions, our scientists co-design assays that align with your research objectives.
| Services | Capabilities |
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| Enzymatic Assays |
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| Mechanism of Action (MoA) |
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| Cellular & Target-Based |
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| Phenotypic Assays |
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HTS-Ready Assays for Hit Finding Campaigns
Looking for high-throughput screening (HTS) solutions? Eurofins Discovery offers HTS-compatible kinase assays using nano volume handling and automated platforms, ideal for:
- Primary screening
- Hit confirmation
- Selectivity & orthogonal testing
Integrated with Genedata Screener® for seamless data analysis
