Monoamine Oxidase B (MAO-B) Human Enzymatic LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Monoamine Oxidase (MAO, E.C.1.4.3.4) catalyzes the removal of an amine group from a variety of substrates including endogenous substances (norepinephrine, epinephrine, dopamine, tyramine, 5-hydroxytryptamine) and many drugs that are amines. MAO functions as an important protective mechanism against exogenous, biologically active amines. There are at least two types of MAO which display dissimilar preferences for substrates and differential sensitivities to selective inhibitors.
Family:
Enzyme
Sub Family:
Catecholamine turnover
Protein Name:
MAO-B
Uniprot Number:
P27338
Gene Name:
MAOB
Gene ID:
4129
Accession Number:
NM_000898
Organism:
Human
Construct Details:
Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Luminescence
Measured Response:
Luminescence
Testing Information
Substrate:
D-Luciferin derivative (4 µM)
Incubation:
60 min at 37°C
Control Inhibitor:
Deprenyl
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 μl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
CNS/Addiction/Pain
Adverse / Beneficial Action:
Selective inhibitors of MAO-A (e.g. clorgyline) appear to have efficacy in the treatment of major depression, and a selective MAO-B inhibitor may have beneficial effect on Parkinsons disease and dyskinesia.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
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